human kidney cancer cell lines caki-1  (Procell Inc)

Journal: FEBS Open Bio

Article Title: MYPT1 inhibits the metastasis of renal clear cell carcinoma via the MAPK8 /N‐cadherin pathway

doi: 10.1002/2211-5463.13487

Figure Lengend Snippet: MYPT1 suppressed ccRCC metastasis in vivo . (A) The lung metastasis model showed that MYPT1 overexpression significantly inhibited ccRCC lung metastasis. Representative gross images of the lungs and bioluminescence images of lung metastases are shown. Arrows indicate pulmonary metastatic nodules. Scale bars: 500 μm. (B, C) Nude mice injected with MYPT1‐overexpressing Caki1 cells had fewer and smaller lung metastases. Representative images of HE staining of the lungs and statistical analysis results are shown. Scale bars: 200 or 50 μm. The pulmonary metastatic nodules were counted under a microscope, and the results were summarized. Data presented as mean ± SEM ( n = 5). Statistical analysis was performed with a two‐tailed unpaired Student's t test (* P < 0.05). ccRCC, renal clear cell carcinoma; HE, haematoxylin–eosin; MYPT1, myosin phosphatase target subunit 1.

Article Snippet: The human kidney cancer cell lines Caki1 and 769P were commercially provided by American Type Culture Collection (ATCC) and cultured as per ATCC's guidelines.

Techniques: In Vivo, Over Expression, Injection, Staining, Microscopy, Two Tailed Test

Journal: FEBS Open Bio

Article Title: MYPT1 inhibits the metastasis of renal clear cell carcinoma via the MAPK8 /N‐cadherin pathway

doi: 10.1002/2211-5463.13487

Figure Lengend Snippet: RNA sequencing and bioinformatic analyses demonstrated that MYPT1 is involved in a MAPK‐related pathway in ccRCC. (A) The volcano plot shows genes differentially expressed between Caki1 cells with MYPT1 overexpression group and the negative control group ( P < 0.05). (B) KEGG analysis of the differentially expressed genes (¦log2FC¦ > 1, FDR‐adjusted P < 0.05) was performed in Caki1‐NC and Caki1‐MYPT1 cells. (C) GSEA revealed that the MAPK signalling pathway gene set was less enriched in Caki1‐MYPT1 cells (FDR < 25% and P < 0.05). (D) KEGG analysis of the MYPT1‐correlated genes ( P < 0.05) in the TCGA‐KIRC dataset. (E) The 17 overlapping genes were enriched in the MAPK pathway based on the differentially expressed genes from RNA sequencing data or MYPT1‐correlated genes from TCGA‐KIRC dataset. ccRCC, renal clear cell carcinoma; GSEA, Gene Set Enrichment Analysis; KEGG, Kyoto Encyclopedia of Genes and Genomes; MAPK, mitogen‐activated protein kinase; MYPT1, myosin phosphatase target subunit 1.

Article Snippet: The human kidney cancer cell lines Caki1 and 769P were commercially provided by American Type Culture Collection (ATCC) and cultured as per ATCC's guidelines.

Techniques: RNA Sequencing, Over Expression, Negative Control

Journal: Disease Markers

Article Title: High Expression of JMJD4 Is a Potential Diagnostic and Prognostic Marker of Renal Cell Carcinoma

doi: 10.1155/2021/9573540

Figure Lengend Snippet: JMJD4 promoted cell proliferation, colony formation, and invasion in kidney cancer cells. (a, b) JMJD4 expression was validated by western blot and qRT-PCR assay. ∗∗∗ indicates a p value < 0.001. (c) Overexpressed JMJD4 promotes the invasion and colony formation capability of Caki-1 cells. ∗∗∗ indicates a p value < 0.001. (d) CCK-8 proliferation curve of Caki-1 cells. ∗∗∗ indicates a p value < 0.001.

Article Snippet: Human kidney cancer cell line (Caki-1), purchased from American Tissue Culture Collection (ATCC; Manassas, VA, USA), were cultured in McCoy's 5A (HyClone, Logan, UT, USA) medium at 37°C, 5% CO 2 in a humidified environment.

Techniques: Expressing, Western Blot, Quantitative RT-PCR, CCK-8 Assay